- Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death Thu, 17 Jun 2021 06:00:00 -0400
Pyrethroids are one of the few classes of insecticides available to control Aedes aegypti, the major vector of dengue, chikungunya, and Zika viruses. Unfortunately, evolving mechanisms of pyrethroid resistance in mosquito populations threaten our ability to control disease outbreaks. Two common pyrethroid resistance mechanisms occur in Ae. aegypti: 1) knockdown resistance, which involves amino acid substitutions at the pyrethroid target site-the voltage-gated sodium channel (VGSC)-and 2)...
- Neonate with chikungunya Thu, 17 Jun 2021 06:00:00 -0400
Chikungunya although rare should be considered in any neonate presenting with fever and facial hyperpigmentation or encephalopathy especially in endemic areas.
- Integrative analysis in toxicological assessment of the insecticide Malathion in Allium cepa L. system Wed, 16 Jun 2021 06:00:00 -0400
For many centuries human populations have been suffering and trying to fight with disease-bearing mosquitoes. Emerging and reemerging diseases such as Dengue, Zika, and Chikungunya affect billions of people around the world and recently has been appealing to control with chemical pesticides. Malathion (MT) is one of the main pesticides used against mosquitoes, the vectors of these diseases. This study aimed to assess cytotoxicity and mutagenicity of the malathion for the bioindicator Allium cepa...
- Clinical features of patients with Rocky Mountain spotted fever, dengue and chikungunya infection Mon, 14 Jun 2021 06:00:00 -0400
CONCLUSION: Rash on the palms and soles, edema and absence of pruritus, together with high levels of direct bilirubin and severe thrombocytopenia could be useful indicators to differentiate patients at RMSF advanced stages from those with dengue and chikungunya.
- Chikungunya virus envelope protein E2 provides a vector for targeted antigen delivery to human dermal CD14(+) dendritic cells Sun, 13 Jun 2021 06:00:00 -0400
- An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses Fri, 11 Jun 2021 06:00:00 -0400
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including Chikungunya, Dengue, and Zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and quantification of Chikungunya, Dengue, and Zika viruses. Amplification assays were performed to verify the construct...
- Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae) Fri, 11 Jun 2021 06:00:00 -0400
CONCLUSIONS: Our findings indicate that L-CWDE produced by T. asperellum reflect chitinolytic enzymes with greater specificity for L3 larval biomolecules. This specificity is characterized by the high percentage of mortality compared with CWDE treatments and also by abrupt changes in patterns of the cellular structures visualized by scanning and transmission electron microscopy. These mixtures of chitinolytic enzymes could be candidates, as adjuvant or synergistic molecules, to replace...
- Facile method for delivering chikungunya viral replicons into mosquitoes and mammalian cells Fri, 11 Jun 2021 06:00:00 -0400
Reverse genetics is an important tool in the elucidation of viral replication and the development of countermeasures; however, these methods are impeded by laborious and inefficient replicon delivery methods. This paper demonstrates the use of a baculovirus to facilitate the efficient delivery of autonomous CHIKV replicons into mosquito and mammalian cells in vitro as well as adult mosquitoes in vivo. The efficacy of this approach was verified via co-localization among an eGFP reporter, nsP1,...
- Challenges towards serologic diagnostics of emerging arboviruses Thu, 10 Jun 2021 06:00:00 -0400
BACKGROUND: Appropriate laboratory diagnostics for emerging arboviruses are key for patient management, surveillance, and intervention, including molecular tests and serologic tests detecting viral antigen or virus-specific antibodies.
- Oral manifestations in chikungunya patients: A systematic review Thu, 10 Jun 2021 06:00:00 -0400
CONCLUSIONS: There seems to be a predominance of oral manifestations that cause discomfort when chewing, such as ulcerations in the acute phase of the disease, with complete remission within 3 to 10 days after the onset, apparently mostly affecting women and older persons. These oral manifestations can be compatible with basic viral infections related to inflammatory response and transitory immunosuppression.